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和平精英游戏创意工坊团队竞技4v4怎么玩:ELISA 配對試劑盒

  熱門產品
酶聯免疫吸附測定 (ELISA) 主要用于樣本中的蛋白質、抗體、多肽或激素的檢測和定量。它是將已知的抗原或抗體吸附在固相載體表面,使酶標記的抗原抗體反應在固相表面進行的技術。之后使用檢測一抗,與抗原形成復合物。如檢測抗體與酶結合,可直接用于抗原定量,或通過另一種酶結合二抗進行定量。方法選擇取決于您所要研究的目標物。



ELISA 法類型:
  • 直接ELISA:
    抗原被固定而酶結合一抗用于檢測或定量抗原濃度。一抗的特異性非常重要。
    • 優點:步驟簡單;可避免來自二抗的交叉反應活性。
    • 缺點:要求對所有一抗進行標記 - 高成本;并非每種抗體都適合進行標記。

  • 間接ELISA:
    一抗不進行標記,對酶結合二抗進行檢測,從而識別一抗。
    • 優點:二抗可進行信號擴增;多種現有二抗可用于不同的分析方法;一抗未經標記,可最大限度保留其免疫反應活性。
    • 缺點:可能出現交叉反應活性。

  • 夾心ELISA:
    待測抗原被結合在捕集抗體層和檢測抗體層之間。兩種抗體必須慎重選擇,以避免出現交叉反應活性或結合位點競爭。
    • 優點:靈敏、高度特異性、抗原在使用前無須純化。
    • 缺點:抗原必須至少包含兩個抗體結合位點。

  • 競爭ELISA:
    來自樣本的目標抗原和經過純化的抗體化抗原與捕集抗體競爭結合。與單獨采用純化抗體時的分析結果比較,如信號強度降低,則表示樣本中存在抗原。
    • 優點:可使用粗(不純)樣本,重現性好。
    • 缺點:總靈敏度降低,總特異性降低。


 
抗體產品展示

 
Antibody Pair for ELISA   PEG Matched Antibody Pair
Antibody pairs for quantitative detection of proteins. Antibody pair to detect and quantify PEGylated compounds.
 
Angiogenesis ELISA Kits   Apoptosis ELISA Kits
ELISA kits for angiogenesis research. ELISA kits for studying programmed cell death in multicellular organisms.
 
Autoimmune ELISA Kits   Binding ELISA Kits
ELISA kits for analysis of autoimmune diseases. ELISA kits for quantitative detection of binding proteins.
 
CD Marker ELISA Kits   Cell Ad/Junc/Cytoskel ELISA Kits
ELISA kits to identify and investigate cell surface molecules. ELISA kits for analysis of cell adhere/junction and cytoskeleton.
 
Cell Cycle ELISA Kits   Cytokine ELISA Kits
ELISA kits for quantitative detection of cell cycle processes. ELISA kits for analysis of cytokine proteins.
 
Diabetes ELISA Kits   Down-Syndrome ELISA Kits
ELISA kits for diabetes research. ELISA kit for investigating Down Syndrome.
 
Enzyme ELISA Kits   Immunoglobulin ELISA Kits
ELISA kits for analysis of different enzymes. ELISA kits for analysis of different immunoglobulins.
 
Infectious ELISA Kits   Interleukin ELISA Kits
ELISA kits to investigate infectious diseases. ELISA kits for analysis of interleukins.
 
Membrane ELISA Kits   Metabolism ELISA Kits
ELISA kits associated with membrane proteins. ELISA kits targeting metabolism.
 
Neurobiology ELISA Kits   Plasma/Serum ELISA Kits
ELISA kits for neurobiology related research. ELISA kits for analysis of proteins in plasma and serum.
 
Signal Transduction ELISA Kits   Small Molecule ELISA Kits
ELISA kits for investigating signal transduction pathway. ELISA kits for analysis of small molecules.
 
Stem Cell ELISA Kits   Other ELISA Kits
ELISA kits for stem cell research. ELISA kits for a wide range of miscellaneous proteins.
 
 
AbVideo™

 
Indirect ELISA

和平精英游戏视频 www.vstuy.icu The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample. In the assay, the antigen of interested is immobilized by direct adsorption to the assay plate. Detection of the antigen can then be performed by using a matched set of primary antibody and conjugated secondary antibodies.


Publish: 2010/04/26 Time: 4:15

 
Sandwich ELISA

The Sandwich ELISA measures the amount of analyte between capture antibody and detection antibody. The analyte needs to have two different epitope sites available for antibody binding.


Publish: 2010/02/12 Time: 3:40

 
Sandwich ELISA-Isotype Detection

Sandwich ELISA is performed to determine the amount and serological class of antibodies made by an immunized animal or present in the serum of patients. The anti-immunoglobulin antibodies used have high specificity and sensitivity.


Publish: 2010/06/30 Time: 3:50

 
Competitive ELISA

In competitive ELISA, unlabeled antibody is incubated in the presence of its antigen. Then these bound antibody/antigen complexes are then added to an antigen coated well. After washing, unbound antibody is removed. The more analyte in the sample, the less antibody will be able to bind to the antigen in the well. The signal is then detected using a labeled secondary antibody and the decrease in signal is compared to a control. The major advantage of a competitive ELISA is the ability to use crude or impure samples and still selectively bind any antigen that may be present.


Publish: 2010/05/03 Time: 3:22

 
 
文獻

[小冊子] Antibody Pairs for ELISA, IP-WB, Protein Phosphorylation, Protein-Protein Interaction.
[小冊子] ELISA and Assay Kits.
[傳單] Antibody Pair for ELISA.
[傳單] Antibody Pair for PEG.
 
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